Microfilaments and FSH stimulation of rat granulosa cell steroidogenesis in vitro
نویسندگان
چکیده
منابع مشابه
Signaling Mechanism of Fsh and Tgfβ1 in Stimulating Steroidogenesis in Rat Ovarian Granulosa Cells
Previous studies demonstrate that transforming growth factor β1 (TGFβ1) augmented FSH-induced progesterone production in rat ovarian granulosa cells. The post-receptor signaling relaying FSH action rests mainly on cAMP followed by protein kinase A (PKA)-dependent and/or PKA-independent pathway possibly involving phosphatidylinositol-3-kinase (PI3K). This study was conducted to investigate the r...
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Connexin 43 (Cx43)-mediated gap junctional communication in granulosa cells is crucial for germ line development and postnatal folliculogenesis. We previously showed that follicle-stimulating hormone (FSH) promoted phosphorylation of Cx43 in rat primary granulosa cells. We further identified Ser365, Ser368, Ser369, and Ser373 in the carboxy-terminal tail as the major sites of phosphorylation by...
متن کاملRole of ERK1/2 in FSH induced PCNA expression and steroidogenesis in granulosa cells.
Follicular development is characterized by both proliferation and differentiation of granulosa cells (GCs) under the control of FSH. However, the cellular mechanism by FSH is not known. Using cultured GCs, we examined whether FSH activated ERK1/2 was involved in the regulation of the proliferation related gene proliferating cell nuclear antigen (PCNA) and steroidogenesis. GCs were obtained from...
متن کاملEffects of fenvalerate on steroidogenesis in cultured rat granulosa cells.
OBJECTIVE This study was designed to examine the in vitro effects of fenvalerate on steroid production and steroidogenic enzymes mRNA expression level in rat granulosa cells. METHODS Using primary cultured rat granulosa cells (rGCs) as model, fenvalerate of various concentrations (0, 1, 5, 25, 125, 625 micromol/L) was added to the medium for 24 h. In some cases, optimal concentrations of 22(R...
متن کاملInteractions of thyroid hormone and FSH in the regulation of rat granulosa cell apoptosis.
Thyroid hormone (TH) is important for normal reproductive function. Our previous studies indicate that FSH increases preantral follicle growth in vitro, a response markedly enhanced by triiodothyronine (T3). However, the nature of this hormonal interaction is poorly understood. The objective of this study was to determine if and how T3 modulate FSH-induced expression and actions of granulosa ce...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1988
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0830263